Journal: Nature immunology
Article Title: Subsets of ILC3-ILC1-like cells generate a diversity spectrum of ILCs in human mucosal tissues.
doi: 10.1038/s41590-019-0425-y
Figure Lengend Snippet: a, The viSNE analysis of tonsil NKp44+ cells based on 36 selected markers and colored by intensity of expression. Each plot shows expression of the indicated cell surface marker. Populations corresponding to ILC3a, ILC3b, ILC1b and ILC1a are delineated in the CD127 plot. Cell subsets that lack CD103, but express CD94, NKG2A and CD127 are indicated as “Others”. b, Heat maps show graded expression of the indicated markers in the ILC subsets. Two donors analyzed in parallel on the same day are shown for consistency. c, The viSNE map of NKp44+CD103+ cells shows 3 clusters in which the degree of expression of CD127 and CCR6 inversely correlates with that of CD94. The viSNE analysis also identifies a small ILC1 cluster expressing CD16. Data are representative of six donors. Cells were gated based on NKp44 and CD161 (a,b) or NKp44, CD161 and CD103 (c) before running viSNE. Contaminating CD3+CD19+FceR+TREM1+CD14+ cells were excluded by manual gating.
Article Snippet: All the following antibodies were purchased from Fluidigm and included in the panel CD45–089Y (3089003B); CD196–141Pr (3141003A); CD19–142Nd (3142001B); CD117–143Nd (3143001B); CD4–145Nd (3145001B); CD8a-146Nd (3146001B); CD25–149Sm (3149010B); FceR-150Nd (3150027B); CD138–150Nd (3150012B); CD103–151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3–154Sm (3154003B); CD85j-156Gd (3156020B); CD194–158Gd (3158032A); CD161–159Tb (3159004B); CD39–160Gd (3160004B); CD27–162Dy (3162009B); CD45RO-165Ho (3165011B); CD34–166Er (3166012B); CD127–168Er (3168017B); CD159–169Tm (3169013B); CD45RA-170Er(3170010B); CD226–171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94–174Yb (3174015B); CD14–175Lu (3175015B); CD56–176Yb (3176009B) and CD16–299Bi (3209002B).
Techniques: Expressing, Marker